Scheme 1. Relationship between biosynthetic gene clusters and the structures of known compounds in various fungi (only partial gene clusters are shown). The ACE1 gene cluster (MGG_08381 – MGG_08391, MGG_12447)6 is shown and the ACE1/RAP1 gene pair which show significant homology to other genes known to produce bioactive compounds are highlighted. The top panel shows key chemical steps catalysed by the fungal PKS-NRPS TENS, which synthesises pretenellin A 1 in partnership with the trans-acting ER TENC. KS, β-ketoacyl synthase; AT, acyl transferase, DH, dehydratase; ER, enoyl reductase; ERO, defective enoyl reductase; CMeT, C-methyl transferase; KR, β-ketoacyl reductase; ACP, acyl carrier protein; C, condensation; A, adenylation; T, thiolation; DKC, Dieckmann cyclase; R, thiolester reductase.
