Fig 1.

MtDNA depletion and reversible impaired immune functions in THP-1 cells. A and B, Treatment with 50 ng/mL ethidium bromide (EtBr) for 8 weeks. A, MtDNA levels. B, LPS-induced TNF-α and IL-8 release. C-E, Transfection with 30 nmol/L negative or TFAM siRNA for 8 days. C, TFAM protein relative to β-actin. D, MtDNA levels. E, LPS-induced TNF-α and IL-8 release. F-H, TFAM recovery 8 days after removal of TFAM siRNA. F, TFAM protein relative β-actin. G, MtDNA levels. H, LPS-induced TNF-α and IL-8 release. All experiments are presented as means ± SD of 3 to 4 independent biological replicates. *P < .05, **P < .01, and ***P < .001.