Fig E4.

Treatment with IFN-γ increases TLR-4 expression and restores immune functions in THP-1 cells with mtDNA depletion following transfection with TFAM siRNA. THP-1 cells were treated with 100 ng/mL recombinant human IFN-γ or medium for the final 24 hours of an 8-day transfection with negative control or TFAM siRNA, or incubation with growth medium. A, Cell viability. B, Phagocytosis of E coli. C, Cell surface expression of TLR-4. All experiments were carried out on 3 independent biological replicates and are presented as means ± SD. ***P < .001.