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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Cell Immunol. 2017 Jul 10;319:28–34. doi: 10.1016/j.cellimm.2017.07.002

Figure 1. Sequence and expression of mutant CD28 proteins.

Figure 1

A) The amino acid sequences of wild type human CD28 and the CD28-F51V and –F51I mutations are shown, with the point mutations bolded. The CTLA4-CD28 chimeric construct consists of the extracellular domain of murine CTLA-4 (aa 1-162 of CTLA4) fused to the transmembrane and cytoplasmic domain of murine CD28 (aa 152-219 of CD28) Amino acid numbering includes the signal peptide. B) CD28-deficient splenocytes were transduced with either empty vector (GFP-RV), or constructs encoding wild type or mutant CD28 constructs and stained with fluorescently conjugated antibodies against CD4 and either CD28 or CTLA-4. CD4/GFP double positive cells were gated on and expression of CD28 or CTLA-4 shown. Representative data of > 5 independent experiments is shown.