Fig. 8. Average Read Coverage of FStitch active calls.
FStitch active calls on the positive strand that completely contain a RefSeq annotation were used to calculate the average behavior. Blue and red represent positive and negative strand coverage, respectively. For each active region, the length was divided into 100 uniformly sized proportions and the read coverage was averaged within each bin. The average annotated 3′ end is noted by the line and transcription beyond the annotation is shaded. Here, we require an FStitch to completely overlap a RefSeq annotation and the RefSeq annotation overlap at least 75% of the FStitch call.