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. 2017 Oct 4;1(2):86–100. doi: 10.1002/jbm4.10015

Figure 2.

Figure 2

Osteoblast CM does not increase myogenic differentiation. (A) Representative fluorescence images of DAPI‐stained and MHC‐stained C2C12 myoblasts after 3 days in DM media (control), 10% 2T3 CM, and 10% MC3T3‐E1 CM. (B) Summary data for FI of control, 2T3 CM, and MC3T3‐E1 CM treatment. The treatment of 10% 2T3 CM and 10% MC3T3‐E1 CM had no significant effect on myogenic differentiation compared to the control (n = 3–5, compared to control, 10% 2T3 CM, p = 0.670; 10% MC3T3‐E1 CM, p = 1.000). Scale bar = 100 μm.