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. Author manuscript; available in PMC: 2017 Nov 2.
Published in final edited form as: Cell Rep. 2017 Jul 11;20(2):491–504. doi: 10.1016/j.celrep.2017.06.060

Figure 2. Maternal peripartum exposure to cefoperazone leads to aberrant development of the host immune system in IL-10 KO mice.

Figure 2

(A) Real-Time qPCR mRNA levels of cytokine and pro- vs. anti-inflammatory genes involved in colonic inflammation from colonic mucosal scrapings in non-treated (NT) vs. cefoperazone (CPZ) male IL-10 KO mice at 3 wks of age. mRNA levels expressed as ΔΔCT relative to housekeeper gene Gapdh. (B) Flow cytometric analyses of live CD45+TCRβ+CD4+ T cells expressing Foxp3+ (Treg), T-bet+ (Th1) or RORγt+ (Th17) in MLNs and colonic LPs of NT vs. CPZ males at 3 wks of age. Data represent percentage of live CD4+ cells. (C) Protein levels of inflammatory cytokines in MLNs of NT vs. CPZ-exposed males at 23 wks of age determined via ELISA. N=4–5/group. NT (black circles), CPZ (black squares). *p<0.05, **p<0.01 via Mann-Whitney U-test. Data are represented as mean±SEM. Female data shown in supplemental data. See also Figure S2.