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. Author manuscript; available in PMC: 2018 Jul 27.
Published in final edited form as: Cell. 2017 Jul 27;170(3):564–576.e16. doi: 10.1016/j.cell.2017.06.010

Figure 5. UBB/UBC as a paralog deficiency MDP in ovarian cancer cell lines.

Figure 5

(A) UBC dependency scores (x-axis) versus UBB expression levels (y-axis). UBB expression (y-axis) versus promoter methylation (x-axis; Fraction) in (B) ovarian cell lines (CCLE data) and (C) tumors (TCGA data). (D) GFP viability competition assay in UBBlow and UBBhigh ovarian cell lines using 4 shRNAs targeting UBC. Log2 fold change of shUBC expressing cells relative to negative controls is shown. (E) Time course of relative viability upon UBC suppression with or without ectopic expression of monoubiquitin (UBB) in a UBBlow cell line (SNU8). Data represent fold change relative to day 1 normalized to pLKO_TRC005-nullT. Error bars represent SD. (F) Levels of conjugated ubiquitin upon UBC suppression in UBBlow (SNU8) and UBBhigh (TOV112D) cell lines.