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. Author manuscript; available in PMC: 2018 Jan 11.
Published in final edited form as: Nat Biomed Eng. 2017 Jul 11;1:0096. doi: 10.1038/s41551-017-0096

Figure 1.

Figure 1

An endothelial cell culture system identifies environments that enable endothelial network formation on synthetic PEG-based hydrogels. (A) Schematic of endothelial cell seeded on synthetic hydrogels and Matrigel. (B) Thin hydrogel arrays assembled with 3-well ProPlate® Cell Culture Isolators. Hydrogel spots were stained using Brilliant Blue for visualization. (C) Left: Endothelial network formation on synthetic hydrogels and Matrigel over time. Right: Typical endothelial networks formed on synthetic hydrogels and Matrigel between 1 and 18 hours after seeding. (D) Qualitative scoring system for HUVEC network formation and heat map showing initial screening results for HUVEC and iPSC-EC network formation. Arrowheads denote environments that enabled HUVEC network formation within 24 hours after seeding and maintained structural integrity over the 48-hour culture period (n = 3). The initial screen was performed once over the course of these studies. Red: Cell Tracker Red.