Fig 5. Real-time RT-PCR results from sampled fish.

Average load of Tenacibaculum spp. positive fish for each sampled group measured with the Tb tuf real-time RT-PCR assay. Dataset includes both randomly sampled fish from each pen at site 2 (n = 30) and moribunds (-M) from each affected pen (n = 10 for site 1 and n = 5 for site 2). The prevalence in the moribunds was 100% and the prevalence in the randomly sampled fish is indicated as % above the respective bar. Striped columns are spring smolts and non-striped columns are autumn smolts. Red columns were treated with florfenicol prior to sampling.