Table 1. Real-time RT-PCR assays.

Assay	Assay Efficacy	Primer/Probe	Sequence
Tb tuf	1.9397	Forward	AGTGTGACGTCCACCTT
		Reverse	CTGTAAGCCAGGTTCTGT
		Probe	TTTCAATACATACACCTCAGC
Mv ompA	1.8826	Forward	GATGATAACGCAACAGCAG
		Reverse	CGGAAACTTACACCAGATAATG
		Probe	TCTTGGAGCAGGTCTAGAATATACACCAG
SAL	1.8501	Forward	GGGAAATCTGTCCGCTTAACG
		Reverse	CCGGTCCCAAGCTGAACA
		Probe	AGGCGTCCAGCGGA
ELF1A	1.9800	Forward	CCCCTCCAGGACGTTTACAAA
		Reverse	CACACGGCCCACAGGTACA
		Probe	ATCGGTGGTATTGGAAC

The sequences of the primers and probes and the efficacy of each real-time RT-PCR assay used in this study [43–45].