Fig 2. Measurement of HeLa cell viability following combination treatment.

MTT was used to measure cell viability 24 hours after each of several treatment conditions; 2-deoxy-D-glucose (2DG), non-radioactive 2-deoxy-2-fluoro-D-glucose (¹⁹FDG), or 1μg/ml doxorubicin alone or in combination, under normoxia or hypoxia. OD readings are presented as a percentage of the OD from untreated control cells. n = 4. a, denotes significance from Dox+2DG, b denotes significance from Dox alone, c denotes significance from normoxia within that treatment, and d denotes significance from 2DG and ¹⁹FDG alone. p≤0.05.