Table 1. Chimeric mice from bone marrow transplantation.
Host | BM donor | BM-Mac | Microglia | Pericytes and OPCs | Line designation | EGFP+ WBC (%) | EGFP+ CD18+ (%) |
---|---|---|---|---|---|---|---|
WT | EGFP+ WT | NG2 + | NG2 + | NG2 + | WT-WTBM | 97.46 ± 3.7 | 94.69 ± 3.5 |
KO | EGFP+ WT | NG2 + | NG2 - | NG2 - | KO-WTBM | 95.57 ± 4.6 | 92.43 ± 2.4 |
WT | EGFP+ KO | NG2 - | NG2 + | NG2 + | WT-KOBM | 95.99 ± 3.5 | 94.14 ± 2.9 |
Transplantation of EGFP bone marrow (BM) from wild type (WTBM) and NG2 null (KOBM) donors into irradiated wild type (WT) and NG2 null (KO) recipients was used to generate 3 lines of chimeric mice. The efficiency of bone marrow engraftment was determined as described in Materials and Methods (Bone Marrow Transplantation). Values shown here represent the percentages of white blood cells (WBC) that are EGFP-positive in each of the chimeric lines. Of importance for the current work, similar EGFP engraftment efficiency is seen for circulating myeloid cells using the β2 integrin marker CD18.