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. 2017 Oct 23;13(10):e1006682. doi: 10.1371/journal.ppat.1006682

Fig 1. L194P is an egg-adaptive substitution in human H3N2 HA.

Fig 1

(A) Frequency of different amino acids observed at HA residue 194 of human H3N2 isolates. Egg: isolates that were sequenced after passaging in egg. Unpassaged: isolates that were sequenced without passaging. (B) Egg-passaged human H3N2 isolates are categorized based on the number of passage. The fractions of isolates in different number of passage that possess a Pro at residue 194 are shown. Error bars represent the standard error estimated from a binomial distribution. (C) Frequency of different amino acids observed at HA residue 194 of pandemic H1N1 (“swine influenza”) human isolates. Egg: isolates that were sequenced after passaging in egg. Unpassaged: isolates that were sequenced without passaging. Of note, no sequence information was found for unpassaged isolates in 2013. (D) Cα traces of the HA structures of Bris07 P194 (blue) and L194 (green) are aligned and compared.

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