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Overview of the reaction scheme. RgNanH hydrolyses α2,3-linked-Neu5Ac on fetuin, releasing 2,7-anhydro-Neu5Ac as the major product but also Neu5Ac. Neu5Ac is further degraded into N-acetylmannosamine (ManNAc) and pyruvate by a sialic acid aldolase, which is inactive against 2,7-anhydro-Neu5Ac.
