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. 2017 Nov 8;451:110–117. doi: 10.1016/j.carres.2017.08.008

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© 2017 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 3 — (A) NMR spectra (600 MHz) of MEME RgNanH-catalysed reaction with standards of Lac, Neu5Gc and Neu5Gcα2-3Lac in overlay in the same buffer conditions (20 mM sodium phosphate, pH 6.5) without BSA. The control is the reaction incubated overnight in the same conditions as the MEME reaction (including BSA) but without enzyme present. (B) ESI(-)-MS spectra of the MEME RgNanH-catalysed reaction with bovine submaxillary mucin without any purification step showing the production of 2,7-anhydro-Neu5Ac (m/z = 290.1) and 2,7-anhydro-Neu5Gc (m/z = 306.1).