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Relative quantification of CALCB gene in pig sacral dorsal root ganglia (A) and spinal cord (B) neurons using the calculated geometric mean of different combinations of reference genes for optimal normalisation as determined by GeNorm (1), BestKeeper (2) and NormFinder (3) software. Data are presented as mean fold change in gene expression ± SD (n = 4 samples/time point). Fold-change expression values for T + 4, T + 8 and T + 16 weeks contrasted against T + 1 week time point.
