Figure 5. MPC expression is reduced in adipose tissue by HF diet and restored by TZD treatment.
[A] The expression of Mpc1 and Mpc2 in epididymal adipose tissue of mice fed LF, HF, or HF containing PIO or MSDC-0602 diets is shown. [B] Representative western blots for MPC1, MPC2, UCP1, and tubulin in epididymal adipose tissue of mice fed LF, HF, or HF containing PIO (HF+P) or MSDC-0602 (HF+M) diets is shown. Average band intensity is shown in graphs below. [C] The expression of Ucp1 mRNA (left) and UCP1 protein (right) is quantified in epididymal adipose tissue of mice treated as above. [For all panels] Values are presented as the mean ± SD. N=6 per group, * indicates p<0.05 vs LF-fed group. † indicates p<0.05 vs HF-fed group. 1 indicates p<0.05 vs comparable WT group.