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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: J Immunol. 2017 Oct 4;199(9):3326–3335. doi: 10.4049/jimmunol.1700534

FIGURE 5.

FIGURE 5

IL-40 is highly expressed in BM stroma and is required for normal B cell homeostasis. (A) BM staining of different cell compartments: stromal cells (CD45−), leukocytes (CD45+), the B cell compartment (CD45+B220+), the myeloid compartment (CD45+CD11b+), and T cell progenitors (CD45+CD11b−). Shown is a representative plot of n = 5 per group, which is representative of two independent experiments. (B) Total cell count for each compartment in (C). Results shown are representative of two independent experiments. (C) qPCR of sorted stromal populations in the BM. Whole BM expression, stromal precursors (Lin−CD45−CD51+), and mature stroma (Lin−CD45−CD51−). Relative expression compared with whole BM. Data are representative of two independent experiments. (D) Number of pre-B cells (CD19+, B220+, IgM−, IgD−, CD43−) in BM of WT and Il40−/− mice. *p < 0.05.