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. 2017 Oct 12;13(10):e1007043. doi: 10.1371/journal.pgen.1007043

Fig 3. Grh, Abd-A and Notch transcriptionally regulate apoptotic enhancer.

Fig 3

(A-D) Shows F3B3-lacZ levels in abdominal pNBs in response to grh (B), abd-A (C) and Notch (D) knockdown compared with pNB blocked for apoptosis by p35 expression (A). (E) Shows the plot of LacZ intensities quantitated and compared from VNCs where abdominal pNBs are blocked from undergoing death by expression of p35 versus VNCs with grh, abd-A and Notch knockdown. (F-G) Comparison of control and Abd-A over expressed larval VNCs with F3-lacZ. (F) Shows basal level of F3-lacZ expression in thoracic NBs. (G) Shows induction of F3-lacZ (white channel) in additional cells in response to ectopic expression of AbdA in thoracic segments of CNS. The dotted lines in these panels enclose abdominal segments of larval VNC which normally express Abd-A. (H) Shows enlarged thoracic region of VNC shown in panel-G. Induction of F3-lacZ and Abd-A in Dpn marked pNB in thoracic segments is shown. The dotted lines in these panels enclose thoracic segments of larval VNC. Yellow arrowheads indicate pNBs. Abdominal and Thoracic segments are indicated as “A” and “T”. Average values are shown as central lines in error bars. Error bars indicate standard deviation.