Figure 6. Histone acetylation level of P27^Kip1 promoter in LY-10 cells.

(A, B) LY-10 cells were grouped into LY-10 (control), LY-10 (vector 1), LY-10 (HO-1), LY-10 (vector 2), and LY-10 (siHO-1). Different groups of LY-10 cells were treated with SAHA (8 μM) or DMSO (0.1%). CHIP-PCR was employed to identify changes in the histone acetylation H3K9 levels at P27^Kip1 promoter in different groups of LY-10 cells. P27^Kip1 promoter sequences in the input DNA that was recovered from antibody-bound chromatin segments were quantified by PCR and normalized to corresponding input controls. P27^Kip1 promoter histone acetylation was examined by agarose gel electrophoresis and quantified with Quantity One software. Data are representative of at least three independent experiments. *P<0.05, **P<0.01.