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. 2017 Jul 28;8(45):78496–78506. doi: 10.18632/oncotarget.19655

Figure 3. TIPE1 arrests cell proliferation and induces apoptosis in lung cancer cell lines.

Figure 3

(A) Assessment of cell cycle by flow cytometry in A549 and H292 cells (n=3; **, p<0.01, compared with Ctrl group). (B) Assessment of cell apoptosis in A549 and H292 cells by Annexin V and PI staining (n=3; **, p<0.01, compared with Ctrl group). (C) Western blotting was performed to detect Cyclin D1 and Cyclin B1 expression in A549 and H292 cells. GAPDH was loading as a loading control. (D) Western blotting was performed to detect caspase 8 and cleaved caspase 3 (CC3) expression in A549 and H292 cells. GAPDH was loading as a loading control.