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. 2017 Sep 11;8(45):78530–78544. doi: 10.18632/oncotarget.20825

Figure 2. Overexpression of RGS4 significantly inhibits melanoma cell proliferation.

Figure 2

(A) Quantitative qRT-PCR result to show RGS4 mRNA significantly increased by pcDNA3.1-RGS4. (B) Western blot of RGS4 expression. (C) Quantification assay of the RGS4 bands intensity. (D) Time course curve of CCK8 assay results to show the viability change of A375 cell. (E) Colony-forming cells evaluated by colony formation assay. (F) Bar charts of positive colonies. (G) The expression of apoptotic-related proteins, Bcl-2, Bax, were detected in A375 cell by Western blot. Error bars indicate ± SD. *p < 0.05; **p < 0.01 by Student’s t-test. All the results were repeated thrice.