Figure 6. Knockdown of RGS4 significantly suppresses cell apoptosis in melanoma cell lines.

(A and B) Comparison of apoptosis ratio of A375 cell transfected with siRGS4 and si-NC by TUNEL assay. (C and D) The expressions of apoptotic-related proteins, Bcl-2, Bax, total Caspase 3 and Cleaved caspase 3, were detected in A375 cell by Western blot. Error bars indicate ± SD. *p < 0.05; **p < 0.01 by Student’s t-test. All the results were repeated thrice.