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. 2017 Sep 11;8(45):78530–78544. doi: 10.18632/oncotarget.20825

Figure 7. RGS4 regulates E2F1 and Cyclin D1 via PI3K/Akt pathway.

Figure 7

(A) Western blot results show expression changes of T-Akt, p-Akt, Cyclin D1 and E2F1 transfected with pcDNA3.1-RGS4 vector and pcDNA3.1 vector in A375 cell. (B) Western blot results show expression changes of RGS4, T-Akt, p-Akt, Cyclin D1 and E2F1 transfected with siRGS4 and si-NC in A375 cell. (C and D) Western blot results show expression changes of RGS4, T-Akt, p-Akt, Cyclin D1 and E2F1 in siRGS4/A375 and si-NC/A375 after treated with PI3K inhibitor LY294002 (30 μM). (E) Model of PI3K/AKT/CyclinD1/E2F1 signaling pathway in melanoma cells. Error bars indicate ± SD. *p < 0.05; **p < 0.01 by Student’s t-test. All the results were repeated thrice.