Figure 3. The LRP-1/β1-integrin complex is not only established in thyroid carcinoma.

Whole-cell extracts were isolated from breast tumor cells (A, left panel), melanoma cells (A, middle panel), glioblastoma cells (A, right panel), COS-7 cells (B, left panel), primary dermal fibroblast (B, middle panel) or endothelial cells (B, right panel). Immunoprecipitation of LRP-1 was conducted and immunocomplexes were immunoblotted using anti-LRP-1 and anti-β1-integrin antibodies as described above. Considering the low level of LRP-1 expression in HUVECs, immunoprecipitations of β1-integrin was favored. Nonspecific IgGs were used as a negative control for each assay.