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. 2017 Sep 5;8(45):78948–78964. doi: 10.18632/oncotarget.20695

Figure 1. BRLF1 accelerates the process of mitosis in NPC cells.

Figure 1

(A) TW01-TetER cells were treated with 50 ng/ml Dox for 24 h and subjected to immunofluorescence staining. (B) TW01-TetER cells were treated with 50 ng/ml Dox and 50 ng/ml nocodazole for 24 h. Nocodazole-arrested TW01-TetER cells were collected by shake-off and released into fresh medium. The samples were collected at the indicated times and cell cycle progression from M to G1 phase was analyzed by flow cytometry. Data are presented as means ± SD. **, P < 0.01, compared to Dox(-) treatment at the same time point. (C) Levels of BRLF1, cyclin B, securin and β-actin were determined by western blot analysis. (D) The samplesat 60 min after nocodazole release were analyzed to determine chromosome-segregation defects. Data are presented as means ± SD.**, P < 0.01, compared to Dox(-) treatment of the same cell.