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. 2017 Aug 24;8(45):78965–78977. doi: 10.18632/oncotarget.20697

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Copyright: © 2017 Hayashi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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CTC isolated from the blood of (A and B) a 38 year old man with a primary refractory localized paraspinal Ewing sarcoma, (C) a 17 year old girl with recurrent osteosarcoma, (D) a 3 year old boy with newly diagnosed alveolar rhabdomyosarcoma, (E) a 9 year old boy with metastatic embryonal rhabdomyosarcoma, and (F) a 23 year old man with localized malignant peripheral nerve sheath tumor. Vimentin is stained red, nuclei stained with DAPI are blue, and CD45 is stained purple. The smaller CD45+ lymphocytes (indicated by yellow arrows in panels A and B) are readily distinguished from larger CD45- sarcoma cells.