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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Clin Cancer Res. 2017 Aug 1;23(21):6567–6579. doi: 10.1158/1078-0432.CCR-17-1574

Fig. 2. AZD9291 stabilizes Bim protein (A) independent of transcription (B), but induces Mcl-1 protein degradation (C and D).

Fig. 2

A and C, The given cell lines were exposed to 100 nM AZD9291 for 8 h followed with the addition of 10 µg/ml CHX. The cells were then harvested at the indicated times for Western blotting to detect the indicated proteins. Band intensities were quantified by NIH image J software and Bim and Mcl-1 levels were presented as a percentage of levels at 0 time post CHX treatment. B and D, The indicated cell lines were pre-treated with 2.5 µg/ml Act D (B) or 10 µg/ml MG232 (D) for 30 min and then co-treated with 100 nM AZD9291 for an additional 4 h. The cells were then harvested for Western blotting.