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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Biopharm Drug Dispos. 2017 Aug 22;38(8):449–457. doi: 10.1002/bdd.2085

Fig. 6.

Fig. 6

Sgk1 phosphorylates Nedd4-2. a. COS-7 cells were transfected with Nedd4-2 and control vector or with Nedd4-2 and CA-sgk1. Transfected cells were lysed and immunoblotted with anti-phospho-Nedd4-2(Ser327)-specific antibody. b. Densitometry plot of results from Fig. 6a, as well as from other experiments. The values are mean ± S.E. (n = 3). c. The same blot from Fig. 6a was re-probed with anti-Nedd4-2. d. Densitometry plot of results from Fig. 6c as well as from other experiments. The values are mean ± S.E. (n = 3). e. The same blot from Fig. 6a, was re-probed with anti-β actin antibody. β actin is a cellular protein marker.