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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Biopharm Drug Dispos. 2017 Aug 22;38(8):449–457. doi: 10.1002/bdd.2085

Fig. 7.

Fig. 7

Sgk1 weakens the interaction between Nedd4-2 and hOAT3. a. Top panel: hOAT3-expressing COS-7 cells were transfected with Nedd4-2 and control vector or with Nedd4-2 and CA-sgk1. Transfected cells were lysed and hOAT3 was immunoprecipitated by anti-myc antibody (epitope myc was tagged to hOAT3), followed by immunoblotting with anti-Nedd4-2 antibody. Bottom panel: The same immunoblot from Fig. 7a, Top panel was reprobed by anti-myc antibody. b. Densitometry plot of results from Fig. 7a, Top panel as well as from other experiments. The values are mean ± S.E. (n = 3).