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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Biopharm Drug Dispos. 2017 Aug 22;38(8):449–457. doi: 10.1002/bdd.2085

Fig. 9.

Fig. 9

Nedd4-2 ligase-dead mutant attenuates the stimulatory effect of CA-sgk1 on hOAT3 transport activity. hOAT3-expressing COS-7 cells were transfected with sgk1 and control vector or with sgk1 and a ligase-dead mutant of Nedd4-2 (Nedd4-2/C821A). 3-min uptake of [3H]-estrone sulfate (0.3 μM) was then determined. Uptake activity was expressed as a percentage of the uptake measured in cells without the transfection of sgk1. Values are mean ± S.E. (n = 3).