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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Mol Cancer Res. 2017 Aug 11;15(11):1503–1516. doi: 10.1158/1541-7786.MCR-17-0136

Figure 2.

Figure 2

Ectopic expression of L858R and ΔE746–E750 EGFR significantly reduces hypoxia associated radiation resistance. Clonogenic survival assay in HBEC cells stably expressing WT (A), L858R (B) and ΔE746–E750 EGFR (C), following a 24 hour exposure to 21% O2 (circles, solid line) or 0.1% O2 (squares, broken lines). Symbols, representing mean SF normalized to PE and error bars representing SD were derived from at least 3 independent experiments, each conducted with samples in triplicate. (D) HRFS values at different SF. Symbols (mean HRFS) and error bars (SD). Asterisk represents summary of an one way ANOVA test performed between WT- and MT-EGFR expressing HBEC cells where p < 0.001. (E) Western blot analysis: Lysates from HBEC cells that were mock transfected (M) or stably transfected with WT-EGFR (WT), L858R (LR) and ΔE746–E750 (DL) forms of EGFR (left panel). Densitometric analysis (right panel) of EGFR band intensities normalized to β-actin band intensities in each lane. Relative to vector alone, levels of WT or MT forms of EGFR were at least 2.5 – 3 orders of magnitude higher