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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Cancer Res. 2017 Sep 18;77(21):5741–5754. doi: 10.1158/0008-5472.CAN-16-3480

Figure 1. Cystathionine-β-synthase (CBS) expression is increased in premalignant polyps.

Figure 1

A) Western blot of protein extracts from freshly collected biopsy specimens probed with antibodies to CBS and cystathionine-γ-lyase (CSE). Under an IRB approved protocol, three polyps were biopsied and diagnosed to be dysplastic polyps by a pathologist [two tubular adenomas (T. Aden.) and one carcinoma in situ (Carc. In situ)]. Three biopsies of “normal” mucosa (N. Muc.) were also obtained from the same patients. B) Densitometry analysis of the Western blot shown in panel A. The bar graph shows mean expression level (with range bars) of CBS [expressed as a ratio of density (AU = arbitrary units) of CBS to β-actin]. Formalin-fixed paraffin-embedded sections of C) normal colonic mucosa, D) hyperplastic polyp, E) tubular adenoma, F) adenocarcinoma and G) normal mucosa immediately adjacent to the adenocarcinoma were stained with a primary antibody to CBS (1:150 dilution). Tissue architecture was visualized by counterstaining with hematoxylin. To assess non-specific staining, serially cut sections were stained with a rabbit isotype-matched (IgG) antibody (dilution 1:150, Santa Cruz Biotechnology, Cat# sc-2763).