Table 1.
Metabolites that are over- or under-expressed in NCM356 cells overexpressing CBS (CBS2) compared to vector-transduced control NCM356 cells.
| Pathway | Biochemical name |
NCM356+CBS NCM356 |
p-value | q-value |
|---|---|---|---|---|
| Alanine and Aspartate Metabolism | N-acetylasparagine | 0.7568 | 0.0023 | 0.0554 |
| Glutamate Metabolism | N-acetylglutamine | 0.8459 | 0.0315 | 0.1203 |
| gamma-aminobutyrate (GABA) | 0.6944 | 0.0035 | 0.0577 | |
| glutamate, gamma-methyl ester | 1.3026 | 0.0231 | 0.1047 | |
| Phenylalanine and Tyrosine Metabolism | N-acetylphenylalanine | 0.7508 | 0.0073 | 0.0742 |
| N-acetyltyrosine | 0.6497 | 0.0295 | 0.1157 | |
| Tryptophan Metabolism | Indolelactate | 0.8672 | 0.0437 | 0.1496 |
| serotonin | 2.4659 | 4.49E-06 | 0.0006 | |
| Leucine, Isoleucine and Valine Metabolism | Tiglylcarnitine | 1.2628 | 0.0333 | 0.1235 |
| 2-hydroxy-3-methylvalerate | 0.6267 | 0.0461 | 0.1504 | |
| alpha-hydroxyisocaproate | 0.5781 | 0.0372 | 0.1343 | |
| Methionine, Cysteine, SAM and Taurine Metabolism | S-adenosylhomocysteine (SAH) | 0.6148 | 0.0024 | 0.0554 |
| Homocysteine | 0.5229 | 0.0028 | 0.0554 | |
| Cystathionine | 1.4358 | 0.0164 | 0.1 | |
| Cysteine sulfinic acid | 1.3029 | 0.0462 | 0.1504 | |
| Polyamine Metabolism | N-acetylputrescine | 0.6283 | 0.0063 | 0.0726 |
| Glutathione Metabolism | ophthalmate | 1.4876 | 0.0093 | 0.0829 |
| Gamma-glutamyl Amino Acid | gamma-glutamylalanine | 0.7866 | 0.0021 | 0.0554 |
| gamma-glutamyl-epsilon-lysine | 1.2492 | 0.0146 | 0.0972 | |
| gamma-glutamylvaline | 1.1864 | 0.0096 | 0.0829 | |
| Dipeptide Derivative | anserine | 1.2466 | 0.0175 | 0.1002 |
| Dipeptide | phenylalanylglycine | 1.9195 | 0.0033 | 0.0577 |
| tryptophylglycine | 1.5487 | 0.0274 | 0.1138 | |
| tyrosylglycine | 2.0093 | 0.0045 | 0.0598 | |
| valylglutamine | 1.4499 | 0.0218 | 0.1047 | |
| leucylglutamine | 1.4636 | 0.0029 | 0.0554 | |
| Glycolysis, Gluconeogenesis, Pyruvate Metabolism | Lactate | 1.3496 | 0.0029 | 0.0554 |
| glycerate | 1.7169 | 0.0105 | 0.0842 | |
| Pentose Phosphate Pathway | 6-phosphogluconate | 1.4264 | 0.0025 | 0.0554 |
| Glycogen Metabolism | maltotriose | 1.2892 | 0.0422 | 0.147 |
| Fructose, Mannose and Galactose Metabolism | mannitol/sorbitol | 1.3805 | 0.0019 | 0.0554 |
| Nucleotide Sugar | UDP-glucose | 1.532 | 0.0205 | 0.103 |
| UDP-galactose | 1.5104 | 0.0026 | 0.0554 | |
| UDP-acetylglucosamine/galactosamine | 0.4683 | 0.0106 | 0.0842 | |
| Aminosugar Metabolism | N-acetyl-glucosamine 1-phosphate | 1.4594 | 0.007 | 0.0742 |
| TCA Cycle | succinate | 0.8096 | 0.0184 | 0.1002 |
| Long Chain Fatty Acid | myristoleate (14:1n5) | 1.3327 | 0.0287 | 0.1144 |
| 10-nonadecenoate (19:1n9) | 1.5602 | 0.0489 | 0.1537 | |
| Fatty Acid, Branched | 15-methylpalmitate | 1.5051 | 0.0178 | 0.1002 |
| Fatty Acid Metabolism (Acyl Carnitine) | octanoylcarnitine | 1.64 | 0.0041 | 0.0586 |
| Phospholipid Metabolism | 1,2-dipalmitoyl-GPC (16:0/16:0) | 1.2133 | 0.026 | 0.1129 |
| 1,2-dioleoyl-GPC (18:1/18:1)* | 1.2038 | 0.0401 | 0.1429 | |
| 1-palmitoleoyl-2-oleoyl-GPC (16:1/18:1)* | 1.2242 | 0.047 | 0.1511 | |
| 1-palmitoyl-2-oleoyl-GPE (16:0/18:1) | 1.2722 | 0.0174 | 0.1002 | |
| 1-stearoyl-2-oleoyl-GPE (18:0/18:1) | 1.2325 | 0.0359 | 0.1312 | |
| 1-palmitoyl-2-linoleoyl-GPE (16:0/18:2) | 1.7167 | 0.0127 | 0.0972 | |
| 1-palmitoyl-2-stearoyl-GPC (16:0/18:0) | 1.2002 | 0.0227 | 0.1047 | |
| 1,2-dioleoyl-GPE (18:1/18:1) | 1.3008 | 0.0079 | 0.0742 | |
| 1,2-dilinoleoyl-GPC (18:2/18:2) | 1.2698 | 0.0424 | 0.147 | |
| 1-oleoyl-2-linoleoyl-GPE (18:1/18:2)* | 1.266 | 0.0133 | 0.0972 | |
| 1,2-distearoyl-GPC (18:0/18:0) | 2.0252 | 0.0153 | 0.0995 | |
| Lysolipid | 1-lignoceroyl-GPC (24:0) | 1.2294 | 0.0194 | 0.1014 |
| Plamalogen | 1-(1-enyl-palmitoyl)-2-linoleoyl-GPE (P-16:0/18:2)* | 0.6839 | 0.019 | 0.1014 |
| Glycerolipid Metabolism | glycerophosphoglycerol | 1.2397 | 0.0331 | 0.1235 |
| Sphingolipid Metabolism | sphingomyelin (d18:1/22:1, d18:2/22:0, d16:1/24:1)* | 1.2077 | 0.028 | 0.1138 |
| sphingomyelin (d18:1/21:0, d17:1/22:0, d16:1/23:0)* | 1.4556 | 0.0068 | 0.0742 | |
| sphingomyelin (d18:2/23:0, d18:1/23:1, d17:1/24:1)* | 1.3062 | 0.0281 | 0.1138 | |
| tricosanoyl sphingomyelin (d18:1/23:0)* | 1.3659 | 0.0238 | 0.1059 | |
| glycosyl-N-stearoyl-sphingosine | 1.5483 | 0.0181 | 0.1002 | |
| glycosyl-N-palmitoyl-sphingosine | 1.3352 | 0.022 | 0.1047 | |
| Mevalonate Metabolism | 3-hydroxy-3-methylglutarate | 1.1996 | 0.0448 | 0.1504 |
| Primary Bile Acid Metabolism | glycocholate | 1.5488 | 0.0028 | 0.0554 |
| taurocholate | 1.577 | 0.0107 | 0.0842 | |
| glycochenodeoxycholate | 1.4125 | 0.0081 | 0.0742 | |
| Purine Metabolism | inosine 5’-monophosphate (IMP) | 0.3173 | 0.0015 | 0.0554 |
| 2’-deoxyinosine | 1.3469 | 0.0135 | 0.0972 | |
| N6-succinyladenosine | 1.1581 | 0.0479 | 0.1524 | |
| guanosine 5’- diphosphate (GDP) | 0.6358 | 0.0165 | 0.1 | |
| guanine | 2.0175 | 0.0143 | 0.0972 | |
| Pyrimidine Metabolism | uridine 5’-monophosphate (UMP) | 1.3071 | 0.0459 | 0.1504 |
| beta-alanine | 0.77 | 0.0061 | 0.0726 | |
| ribose 1-phosphate | 1.2259 | 0.0266 | 0.1129 | |
| Nicotinate and Nicotinamide Metabolism | nicotinamide ribonucleotide (NMN) | 1.6278 | 0.0075 | 0.0742 |
| nicotinamide adenine dinucleotide (NAD+) | 1.2129 | 0.0205 | 0.103 | |
| 1-methylnicotinamide | 0.652 | 0.0041 | 0.0586 | |
| adenosine 5’-diphosphoribose (ADP-ribose) | 2.6864 | 1.86E-05 | 0.0017 | |
| Riboflavin Metabolism | flavin adenine dinucleotide (FAD) | 1.3294 | 0.0208 | 0.103 |
| flavin mononucleotide (FMN) | 1.4536 | 0.0042 | 0.0586 | |
| Tocopherol Metabolism | alpha-tocopherol | 1.4689 | 0.0049 | 0.0618 |
| Folate Metabolism | 5-methyltetrahydrofolate (5MeTHF) | 1.3164 | 0.0157 | 0.0996 |
| Thiamine Metabolism | thiamine diphosphate | 0.7367 | 0.0299 | 0.1157 |
| Vitamin B6 Metabolism | pyridoxamine | 1.2353 | 0.0139 | 0.0972 |
| pyridoxal phosphate | 1.3339 | 0.0224 | 0.1047 | |
| Benzoate Metabolism | O-methylcatechol sulfate | 1.4732 | 0.0264 | 0.1129 |
| Chemical | lanthionine | 12.2969 | 1.54E-06 | 0.0004 |