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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: J Cell Physiol. 2017 Jun 6;233(3):1812–1822. doi: 10.1002/jcp.25983

Figure 4. Cardiac marker expression after 7 days of treatment.

Figure 4

Mouse DFAT cells were treated with growth medium supplemented with control vehicle (day 5-7), BMP10 (25 ng/ml, day 5-7), CV2/5-7 (50 ng/ml, day 5-7), BMP10 (day 5-7) followed by CV2 (day 8-10) (BMP10/CV2), anti-BMP10 antibodies (100 ng/ml, day 5-7), or CV2/8-10 (50 ng/ml, day 8-10).

(A) Expression of Nkx2.5 and Troponin I was determined by qPCR.

(B) Proliferation was determined by direct cell counting.

(C) Localization of Nkx2.5 (green) and Troponin I (Tr, red) was determined by immunofluorescence. DAPI (blue) was used to visualize nuclei.

*<0.1, **<0.01 (n=3).