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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: J Cell Physiol. 2017 Jun 6;233(3):1812–1822. doi: 10.1002/jcp.25983

Figure 5. Cardiac marker expression after 14 days of treatment.

Figure 5

Mouse DFAT cells were treated with growth medium supplemented with control vehicle (day 5-7), BMP10 (25 ng/ml, day 5-7), CV2/5-7 (50 ng/ml, day 5-7), BMP10 (day 5-7) followed by CV2 (day 8-10) (BMP10/CV2). Cell morphology and expression of sarcomeric alpha-actinin (Sr-alpha-actinin, green) and Troponin I (Tr-I, red) were examined after 14 days by immunofluorescence. DAPI (blue) was used to visualize nuclei.