Fig. 5.

Effect of pH and temperature on LrXynA activity and stability. a Effect of pH on xylanolytic activity (Black circle) and stability (Black square) of LrXynA. LrXynA was incubated in 50 mM citrate–phosphate (3–7) or phosphates (6–8) buffer and incubated at 50 °C for 10 min; for pH stability, LrXynA was preincubated at 50 °C for 3 h in the same buffers. b Effect of temperature on the xylanolytic activity of LrXynA. The enzyme was incubated in 0.2% (w/v) Beechwood xylan in 50 mM citrate–phosphate buffer, pH 6.0 at different temperatures (30–80 °C). c Thermostability of LrXynA at 75 °C (Black up-pointing triangle), 65 °C (Black square) and 55 °C (Black diamond suit)