Fig. 6.

Serial transmissions of myelopathy and vCJD from macaques to mice. a Primates exposed to blood or cerebral products derived from vCJD-infected macaques developed vCJD (primates R3 and D6) or myelopathy (primates R5 and D7, Supplementary Text 1). Interestingly primate R3 (duplicate of primate R5) developed vCJD with spongiform changes and accumulation of PrP^d in its brain and spinal cord, but it also exhibited myelopathic lesions (pseudo-necrotic lesions in the brainstem and demyelination of posterior tracts) and limited amounts of PrP^d in its brain (around 10-fold lower than the other vCJD primates). b Corresponding blood and brain samples were inoculated intravenously (IV) or intracerebrally (IC) into Swiss mice. The lesions observed in spinal cords of macaques are presented (hemalun–eosine, H&E). The presence of PrP^d in the spinal cords was detected by immunohistochemistry with different monoclonal anti-PrP antibodies (here Sha-31, for other antibodies see Fig. 5) after proteolysis treatment