Figure 2.
In situ hybridization of SlPIN1 in the tomato pedicel during abscission. Images of longitudinal sections of the distal part (a,d,g,j), AZ (b,e,h,k) and proximal part (c,f,i,l) 0 h (a-c), 1 h (d–f), 2 h (g–i) and 4 h (j–l) after flower removal and detection of SIPIN1 using an antisense probe. Sections detected using a sense probe of SlPIN1 (m–o) were used as controls. The insert in d shows a magnified image of the region shown in the white box. Tissues are indicated by white letters. C, cortex, V, vascular bundle, P, pith, AL, abscission layers. Scale bar = 100 μm.