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. 2017 Nov 2;7:14919. doi: 10.1038/s41598-017-15072-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Subcellular localization of the SlPIN1-GFP protein and the phosphomimic SlPIN1-GFP S418D protein. GFP (a,d), bright-field (b,e) and merged (c,f) images were detected using a confocal laser scanning microscopy. (a–c) The basal localization of SlPIN1-GFP on the PM in tomato root cells. (d–f) Nonpolar localization of SlPIN1-GFP S418D on the PM in tomato root cells. The polarity is indicated by the white arrowheads. Scale bar = 50 μm.