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. 2017 Nov 2;7:14919. doi: 10.1038/s41598-017-15072-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Interaction proteins of SlPIN1HL. (a) Structure of SlPIN1. SlPIN1HL was used as Y2H bait. (b) Interactions between SlPIN1HL and ten positive proteins were confirmed using Y2H assay. The ten interaction proteins are indicated by numbers 1 to 10. 1, PREDICTED: Solanum lycopersicum mitochondrial outer membrane protein porin of 34 kDa. 2, Glutamine synthetase. 3, PREDICTED: expansin-like B1. 4, proteasome-like protein alpha subunit-like. 5, PREDICTED: Kunitz-type protease inhibitor B (E3 ubiquitin-protein ligase). 6, PREDICTED: LRR receptor-like serine/threonine-protein kinase FEI 2 isoform X 1. 7, PREDICTED: LysM domain receptor-like kinase 4. 8, protein phosphatase 2 C. 9, ribonuclease T2 precursor. 10, pathogenesis-related protein STH-2. SV40 and P53 were used as positive controls. The AD and BD vectors were used as negative controls. The blue strain indicates the interaction between two proteins. (c) BiFC confirmation of the interaction between SlPIN1HL and PP2C. Fusion proteins of SlPIN1HL-YFPc, PP2C-YFPn, YFPn and YFPc were pairwise-expressed in tobacco leaves. YFPn and YFPc were used as negative controls. Scale bars = 50 μm.