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. 2017 Nov 2;7:14919. doi: 10.1038/s41598-017-15072-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GUS expression in the SlPIN1-VIGS line. (a) GUS staining in the pedicel. GUS staining is observed in the vascular strands (v) and the AZ of the pedicel, and the GUS staining in the Control is much deeper than that in line 1–6, 1–9 and 1–10. (b) GUS staining in the ovary. Strong GUS staining is observed in the ovary wall (o) and the basal tissue of the ovary in line 1–6, 1–9 and 1–10 (arrowheads). Only slight staining is visible in the basal tissue connecting the pedicel in the Control (arrowheads). The IAA levels in the AZ (c) and ovary (d) are shown in histograms. The asterisk indicates significant differences (P < 0.05) according to Duncan’s multiple range test. Flowers were sampled at anthesis. pl, placenta, o, ovary wall. Scale bars = 1 mm.