Figure 2.

Generation of the dsx1 reporter strain. (A) Knock-in approach. See description in the main text. (B) Genotype of the obtained dsx1 reporter strain. At the dsx1 locus, targeted hemizygous knock-in of two tandem copies of the reporter cassette was achieved, leading to the silencing of dsx1 on this allele. Therefore, the genotype at this locus could be depicted as dsx1 ⁺ /mCherry-dsx1 ⁻. Genomic sequences of regions susceptible to in-del mutation after knock-in (left and right junctions, connecting junction of the two donor copies, and TALEN target sequence) are shown. For the reference sequences, an NHEJ was assumed to occur without causing any in-del mutations. Scaffold and contig information were obtained from the “2015 March Daphnia magna Gene-ome early release dataset” (http://arthropods.eugenes.org/EvidentialGene/daphnia/daphnia_magna/). Images are not to scale.