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. 2017 Nov 2;7:13521. doi: 10.1038/s41598-017-13730-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dynamic progression of dsx1-expressing cells during early embryogenesis. This snapshot series of two males and one female of the dsx1 reporter strain was extracted from Supplementary Movie S1, showing major events from gastrulation until thoracic segmentation. From top to bottom are frame 39, 50, 60, 69, 102, and 137 from the movie. Schematic diagrams on the right illustrate simple anatomy of the embryos at the corresponding time points. The diagrams from stage (i) to (iv) show images of embryonic sections. The diagrams (v) and (vi) show superficial views of embryos. a: anterior, p: posterior, v- ventral, d: dorsal, Bp: blastopore, Sp: Scheitelplatten, Sto: stomodeum, Pro: proctodeum, t1–3: thoracic segments, a1: first antenna, a2: second antenna, hpo: hours post ovulation. White arrow heads indicate mCherry-expressing cell clusters that migrate in an anterior-to-posterior direction. An asterisk indicates the site of invagination.