Figure 5.

Effective release of ACF from Biodegradable Wafers in vitro and in vivo. Acriflavine is effectively released for over 100 days from the wafers (A) In vitro kinetic release profiles of 10%, 25% and 50% ACF wafers measured using the fluorescence intensity of the drug. The curves show the amount of drug released in 1 ml vials containing ACF wafers in PBS measured at close time points during the first phase of release from (0.5 to 48 hours) and weekly during the second phase up until 120 days. Mean ± SE of three separate experiments are plotted (n = 3). (B) Ex vivo imaging of the brain implanted intracranially with 25% ACF wafers confirmed the release of drug in vivo. Representative 3D cartoon showing the release of ACF from the intratumoral implanted wafer and the following reduction of the tumor mass (a) At 30 days the fluorescence intensity was higher compared to 60 days when the polymer was almost dissolved as and no tumor is macroscopically visible. The brains of the animals no receiving ACF (control) were fluorescent negative (b).