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. 2017 Nov 3;7:14610. doi: 10.1038/s41598-017-14726-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Post-printing cell viability tests. (a) The LIVE/DEAD assay was used to measure cell survival immediately after printing. Each bar indicates an individual assay (n = 5). (b) The proliferation rate was measured every other day for 7 days. No significant differences were observed using the two-way ANOVA followed by Tukey’s post hoc test, p > 0.05; error bars indicate the s.d. (n = 5). (c) Long-term cell morphology was monitored until passage 8. Cells from both the printed and control groups maintained their original morphologies. Nuclei were stained with Hoechst 33342 dye. Scale bar: 50 μm.