Table 4.

Cell processes identified by sub-network enrichment analysis (SNEA) that were regulated by 1000 nM SNa in primary Carassius auratus RGC cultures.

	Gene Set Seed	Total # in pathway	# of Measured Neighbors	Median change	P-value
Processes related to CNS	Axon guidance	210	160	1.17	0
	Transmission of nerve impulse	598	321	1.1	0
	Cognition	212	126	1.11	0.004
	Innervation	172	113	1.08	0.005
	Brain function	150	89	1.14	0.006
	Cerebellum development	14	8	1.44	0.007
	Neuronal activity	263	133	1.06	0.008
	Neuroproliferation	6	5	1.7	0.01
	Hindbrain development	15	11	1.44	0.013
	Synaptic transmission	509	288	1.13	0.013
	Synaptic plasticity	454	284	1.1	0.013
	Schwann cell formation	15	12	1.26	0.016
	Memory	721	385	1.08	0.016
	Neuron development	103	69	1.1	0.029
	Dentate gyrus development	6	6	1.34	0.03
	Neurogenesis	561	383	1.11	0.031
	Central nervous system function	25	15	1.26	0.041
	Axon extension	74	59	1.16	0.042
	Glial cell development	17	11	1.25	0.044
Processes related to immunity	B-cell activation	233	105	1.1	0.001
	Leukocyte function	158	74	1.09	0.003
	Granulocyte adhesion	52	27	1.11	0.009
	Peritoneal macrophage function	10	5	1.34	0.014
	Immunoreactivity	463	260	1.13	0.014
	Eosinophil degranulation	43	15	1.31	0.015
	T-cell homeostases	100	50	1.05	0.016
	Respiratory burst	185	92	1.1	0.017
	NK cell mediated cytotoxicity	367	133	1.09	0.018
	Leukocyte accumulation	84	43	1.1	0.019
	Leukocyte differentiation	9	5	1.53	0.022
	B lymphocyte proliferation	277	148	1.07	0.022
	Lymphocyte activation	277	131	1.11	0.023
	Lymphocyte aggregation	10	6	1.32	0.027
	Macrophage response	48	19	1.1	0.028
	Immune system activation	163	76	1.07	0.033
	Leukocyte tethering / rolling	106	58	1.11	0.041
	Phagocyte activity	167	80	1.1	0.046
	Granulosa cell differentiation	46	34	−1.13	0.047

Only those sub-networks with a median fold-change greater than 5% in the cell process are presented (P < 0.05). Reported here are only some major cell processes affected by SNa in RGCs.