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. 2017 Nov 3;7:14597. doi: 10.1038/s41598-017-15058-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Methaemoglobin at baseline, after 20 min, and after 140 min of continuous nitrite or saline infusion. Data are mean ± SEM, n = 9 for the saline group (control), n = 11 for the nitrite group, *denotes p < 0.05 control vs. nitrite group, + denotes p < 0.05 vs. baseline in the nitrite group. Both groups were exposed to a brief hypoxic challenge and to unilateral ischemia-reperfusion between the blood sampling at 20 min and that at 140 min.