Fig. 5.

Autophagic degradation of Kenny in HeLa cells depends on a functional LIR motif and its interaction with co-expressed Atg8a. Confocal images of HeLa cells transiently expressing tandem tagged, mCherry-eYFP-Kenny^WT or F7A/L10A (red and green) and Myc-Atg8a. a, b Cells cultured in full media. c, d Cell starved for 5 h in Hanks media to activate autophagy. e, h To inhibit lysosomal degradation, the cells in full media e, f or Hanks media g, h were treated with Bafilomycin A1 (BafA1). Insets on the right of merged channels show the separate red and green channels. Scale bars are 10 and 2 µm (insets)